Preliminary Investigation into the Anti-Tumor Mechanism of Ganoderma lucidum Crude Polysaccharides-tes
DOI:
https://doi.org/10.67206/ch221f89Keywords:
Ganoderma Lucidum Polysaccharide, Cancer-Associated Fibroblasts, Immune Cells, ActivationAbstract
This study aims to explore the potential anti-tumor mechanisms of Ganoderma Lucidum Polysaccharide (GLP). Flow cytometry was employed to investigate the activation effects of GLP on different immune cell subsets. The direct inhibitory effects of GLP on tumor cells were evaluated using CCK assay, MTT assay, and real-time cell monitoring (Cell Index) methods. Additionally, the cytotoxic effects of GLP on cancer-associated fibroblasts (CAF) were examined. A hypothesis was proposed that GLP inhibits tumor development by improving the tumor microenvironment. The results indicate that GLP exerts anti-tumor effects not only by directly killing tumor cells but also by inhibiting the growth of CAFs and primarily promoting CD4+ T cell proliferation.
References
[1] Boh B. Ganoderma lucidum: a potential for biotechnological production of anti-cancer and immunomodulatory drugs[J]. Recent Patents on Anti-Cancer Drug Discovery, 2013, 8(3): 255-287. https://doi.org/10.2174/1574891x113089990036. PMID: 23227951. DOI: https://doi.org/10.2174/1574891X113089990036
[2] Suarez-Arroyo I J, Rosario-Acevedo R, Aguilar-Perez A, et al. Anti-tumor effects of Ganoderma lucidum (reishi) in inflammatory breast cancer in in vivo and in vitro models[J]. PLOS ONE, 2013, 8(2): e57431. https://doi.org/10.1371/journal.pone.0057431. PMID: 23468988; PMCID: PMC3585368. DOI: https://doi.org/10.1371/journal.pone.0057431
[3] Jiao C, Xie Y Z, Yang X, et al. Anticancer activity of Amauroderma rude[J]. PLOS ONE, 2013, 8(6): e66504. https://doi.org/10.1371/journal.pone.0066504. PMID: 23840494; PMCID: PMC3688780. DOI: https://doi.org/10.1371/journal.pone.0066504
[4] Han S, Ma C, Hu M, et al. A polysaccharide from Dictyophora indusiata inhibits the immunosuppressive function of cancer-associated fibroblasts[J]. Cell Biochemistry and Function, 2017, 35(7): 414-419. https://doi.org/10.1002/cbf.3290. PMID: 28990218. DOI: https://doi.org/10.1002/cbf.3290
[5] Zhao H, Zhang Q, Zhao L, et al. Spore Powder of Ganoderma lucidum Improves Cancer-Related Fatigue in Breast Cancer Patients Undergoing Endocrine Therapy: A Pilot Clinical Trial[J]. Evidence-Based Complementary and Alternative Medicine, 2012, 2012: 809614. https://doi.org/10.1155/2012/809614. PMID: 22203880; PMCID: PMC3236089. DOI: https://doi.org/10.1155/2012/809614
[6] Wang P Y, Zhu X L, Lin Z B. Antitumor and Immunomodulatory Effects of Polysaccharides from Broken-Spore of Ganoderma lucidum[J]. Frontiers in Pharmacology, 2012, 3: 135. https://doi.org/10.3389/fphar.2012.00135. PMID: 22811667; PMCID: PMC3395810. DOI: https://doi.org/10.3389/fphar.2012.00135
[7] Sun L X, Lin Z B, Duan X S, et al. Ganoderma lucidum polysaccharides counteract inhibition on CD71 and FasL expression by culture supernatant of B16F10 cells upon lymphocyte activation[J]. Experimental and Therapeutic Medicine, 2013, 5(4): 1117-1122. https://doi.org/10.3892/etm.2013.931. PMID: 23596479; PMCID: PMC3628224. DOI: https://doi.org/10.3892/etm.2013.931
[8] Liu H M, Cheng J, Wang X Y, et al. Structure Identification of Ganoderma lucidum Spore Polysaccharides and Their Antitumor Activity In Vivo[J]. Molecules, 2024, 29(10): 2348. https://doi.org/10.3390/molecules29102348. PMID: 38792209; PMCID: PMC11124482. DOI: https://doi.org/10.3390/molecules29102348
[9] Thyagarajan A, Jedinak A, Nguyen H, et al. Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK)[J]. Nutrition and Cancer, 2010, 62(5): 630-640. https://doi.org/10.1080/01635580903532390. PMID: 20574924. DOI: https://doi.org/10.1080/01635580903532390
