Inhibitory Effects of Ganoderma lucidum Spore Oil on TS/A Breast Cancer Cell Growth
DOI:
https://doi.org/10.67206/pgb14s12Keywords:
Ganoderma lucidum spore oil, TS/A murine breast cancer cells, Apoptosis, Stat1, Tumor microenvironmentAbstract
Objective: To determine whether Ganoderma lucidum spore oil selectively inhibits the growth of TS/A murine breast cancer cells, induces TS/A apoptosis, and modulates apoptosis-related genes. Methods: MTT assays were used to examine the growth responses of normal LX-2 fibroblast-like hepatic stellate cells and TS/A cells. Flow cytometry with propidium iodide staining was used to estimate apoptosis, and real-time quantitative PCR was used to measure the expression of apoptosis-related transcripts. Results: Ganoderma lucidum spore oil markedly inhibited TS/A growth in a concentration-dependent manner. Low concentrations promoted LX-2 growth, whereas high concentrations inhibited it. The expression of Casp8, Casp3, and Stat1 changed after treatment, consistent with induction of apoptosis. Significance: Ganoderma lucidum spore oil may induce apoptosis in TS/A breast cancer cells by altering apoptosis-related gene expression and could provide candidate components for further antitumor drug development.
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